Paul H. Chung, MD, presented “Next Generation DNA Sequencing for Genitourinary Implants” for the Grand Rounds in Urology audience in January 2021.

How to cite: Chung, Paul H. “Next Generation DNA Sequencing for Genitourinary Implants” January 2021. Accessed Jul 2024. https://grandroundsinurology.com/next-generation-dna-sequencing-for-genitourinary-implants​/

Summary:

Paul H. Chung, MD, Assistant Professor and Director of the Division of Reconstructive Urology at the Sidney Kimmel Medical College of Thomas Jefferson University, discusses infection of genitourinary implants and how next-generation sequencing can be used to manage device infection. Presenting his results from a recently published article with the Canadian Journal of Urology, he outlines the methods, outcomes, and recommendations for clinicians, specifically those removing malfunctioning or infected penile prostheses and artificial sphincters. Dr. Chung highlights the benefits of polymerase chain reaction (PCR) and next-generation sequencing (NGS) including faster processing time, greater sensitivity, and the ability to run both fungal and bacterial analysis in the same setting. Additionally, NGS  is useful in the selection of  perioperative antibiotics and irrigation solutions, as well as determining how to coat devices during implant surgery. Following the presentation, Grand Rounds in Urology editor J. Curtis Nickel, MD, FRCSC, interviews Dr. Chung to further discuss the clinical implications of his research. In the conversation, they compare biofilm collection techniques, noting that PCR and NGS are most helpful in a clinical setting, whereas  extended culture is useful in a research setting.

For more information on the urinary microbiome, visit our Next Generation Microbiome & Urologic Infections Learning Center.

ABOUT THE AUTHOR

Dr. Paul H. Chung is an Assistant Professor and Director of the Division of Reconstructive Urology at the Sidney Kimmel Medical College of Thomas Jefferson University in Philadelphia, PA. He graduated with a BS in electrical engineering from Northwestern University before obtaining his medical degree at Thomas Jefferson University. Subsequently, Dr. Chung completed a research fellowship in the National Institutes of Health’s Clinical Research Training Program in Bethesda, Maryland and his general surgery and urology residency training at the University of Texas Southwestern Medical Center in Dallas, Texas. He then completed a clinical fellowship in urologic trauma, reconstruction, and prosthetics at the University of Washington Medical Center in Seattle, Washington. Dr. Chung is an active member of the American Urological Association, American College of Surgeons, Society of Genitourinary Reconstructive Surgeons, Société Internationale d'Urologie, Society of Urologic Prosthetic Surgeons, International Society for Sexual Medicine, and Sexual Medicine Society of North America. He has published over 75 abstracts and manuscripts in a number of peer-reviewed journals. Dr. Chung’s practice is focused on erectile dysfunction, Peyronie’s disease, stress urinary incontinence, urethral stricture disease, buried penis, genital lymphedema, enlarged prostates, low testosterone, and genitourinary reconstruction.